As osmosis is defined as the movement of water molecules across a partially permeable membrane from an area of high water concentration to an area of low water concentration, i predict that as the water concentration decreases in the potato pieces the overall mass and length will decrease too. This is the molarity of a solution that has a water potential equal to the water potential of the potato tuber cells 9 use the table below to work out the water potential of a solution having this molarity. Estimate / determine the water potential of potato tissue equivalent to a particular i made progress on this second lab report our assumption is that potato cells will behave like the sailor's cells in her.
Resource - measuring the water potential of a potato cell including the charadakov method osmosis - effects of solute concentration on potato discs asexual reproduction - potatoes can be used as an example plant for asexual reproduction along with spider plants , the mexican hat plant and strawberries. To determine the water potential of potato tuber tissue and as the solute potential of the external solution increases, as does the mass loss of the potato chips i predict that for my experiment that as i increase the solute potential of the external solution from 0kpa to -1120kpa the mass gain will decrease as the solute potential decreases. Tubers did not become infected at soil depths of 8 and 17 cm incidence of tuber infection when water volumes of 200, 600, 400, 800 and 1000 ml of water were applied to the soil surface was 27, 29, 27, 22 and 22, respectively and were not not significantly different (p 005.
The solution gains or looses water depending on the water potential of the tissue if the density of a solution does not change (no net movement of water) then this solution has the same water potential as the tissues that were incubated in it. The water potential of the potato would decrease because water moves from a high water potential region to a low potential region, and a dehydrated potato cell is hypertonic in comparison with the environment. Project methods objective 1: potato tubers (cv russet burbank) will be grown and harvested at the university of idaho kimberly research and extension center tubers will be cured for 2 weeks at 55 f, and the temperature decreased 05 f/day to a final holding temperature of 45 f relative humidity will be maintained above 95% for the study.
The aim of this investigation is to determine the water potential of potato tissue, by measuring the difference in length, before and after selected cells have been placed in varying molar sucrose solutions, and then calculating the water potential accordingly. Calculate the change in mass and then percentage change 7 plot a graph of your processed data and use this to determine the concentration of sucrose which has the same water potential as the potato tuber cells. Figure 2 - graph showing the relationship between the concentration of sucrose solution and its water potential the solute potential of the potato tuber cells is the same as the solute potential of a sucrose solution in which the potato cylinder did not change in mass. The controlled variables were the use of di water, the amount of time spent in the solution, using tubers from the same potato, using the same length and width of potato tubers, stirring the same amount in every solution, the same volume of liquid in every concentration of sucrose, and weighing each potato tuber to 01 grams.
Calculate the change in mass and then calculate the percentage change in mass - easier to compare as potatoes may have different start masses 9 plot a graph of your processed data and use this to determine the concentration of sucrose which is which has the same water potential as the potato tuber cells - when curve crosses x axis (% change. A method of measuring the water potential of stored potato tubers (solanum tuberosum l) was needed to investigate the relationship of bacterial soft rot in tubers to water potential. The potato weight gain or loss is graphed versus the water potential of the sugar solutions to determine the point where there would be no weight gain or loss at that point the potato water potential would equal the solution water potential. An investigation to find the water potential of potato and carrot tubers in a sucrose solution, of concentration 000 - 050mol, over a 24 hour period interpretation written communication c1 from our graph it can be seen that the concentration of sucrose solution is 018 m at 0% change in mass for the potato and 0355 m at 0% change in mass.
Objective the objective of this lab is to measure the water potential of potato tuber tissues we will monitor water uptake or loss by tissues in different sucrose solutions to identify an isotonic solution with water potential equal to that of the tissue. There are several different methods in determining water potential one of the earliest was done by meyer and wallace which utilized the change in weight method to determine the water potential of white potato tuber between -77 and -83 bars (meyer and wallace. Determination of the water potential of potato tuber cells method five sucrose solutions with varying molarity and one control containing distilled water were prepared and poured into test tubes.